(339) 555-0115 ◇ Cambridge, MA
isolde.tremaine@example.com ◇ linkedin.com/in/isolde-tremaine ◇ isoldetremaine.com
Biotechnology research associate with 6 years in cell and molecular biology at a biotech company developing antibody therapeutics, supporting 3 discovery programmes from target validation to lead selection. Ran about 900 cell-based and biochemical assays a year with a 96% first-pass acceptance rate at data review, developed 4 assays that became programme standards, and contributed data to 2 investigational new drug submissions. Generates data that a decision can rest on, and writes it up so the next scientist can reproduce it.
Thesis on antibody binding characterisation; coursework in Molecular Biology, Protein Engineering, Immunology, and Biostatistics.
Completed a good laboratory practice course in 2021 and a flow cytometry certification in 2023.
- Support 3 antibody discovery programmes from target validation to lead selection at a biotech company of 220, running about 900 assays a year.
- Hold a 96% first-pass acceptance rate at data review across 3 years and contributed data to 2 investigational new drug submissions with 0 data queries from regulators.
- Developed 4 cell-based and binding assays that became programme standards, cutting lead candidate screening time from 6 weeks to 3.
- Ran about 600 binding and functional assays a year for 2 discovery programmes at a biotech company of 80.
- Characterised 120 antibody candidates by surface plasmon resonance with binding data that supported the selection of 3 leads.
- Cut assay variability 40% by standardising cell passage and reagent lot controls across 4 assays.
- Characterised binding kinetics of 40 engineered antibody variants for a thesis project across 2 years.
- Co-authored 1 peer-reviewed paper and presented a poster at a national conference of about 3,000 attendees.
- Trained 4 undergraduate researchers on cell culture and ELISA.
Cell-Based Potency Assay Development. Developed a cell-based potency assay for an antibody programme from a reporter cell line, optimising 6 parameters across 30 experiments and validating precision and linearity, which became the programme standard, cut screening time from 6 weeks to 3, and supported a lead selection decision on 80 candidates.
Investigational New Drug Data Package. Generated and documented the in vitro characterisation data for 2 submissions, running about 150 assays under good laboratory practice principles with full electronic notebook records, which passed internal quality review and drew 0 data queries from regulators.
Assay Variability Reduction. Traced assay variability across 4 assays at a previous employer to cell passage number and reagent lot changes, built a control chart and a lot qualification step, and cut coefficient of variation 40%, which removed about 20 repeat experiments a year.
- Member of a regional biotechnology association, attending about 6 seminars a year.
- Volunteer science mentor for a high school programme, coaching 3 students a year on research projects.
- Judge for a regional science fair with about 150 projects each spring.
- Lead assay development for a discovery biology team of 9 and own 4 programme standard assays.
- Trained 6 research associates on cell-based assays and notebook standards.
- Present assay data at 3 programme team meetings a month with chemists and programme leads.

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